Fastlink Conjugation Kits for One-Step Antibody Labelling

• One-step process

• 30 seconds hands-on time

• Reproducible

• Scalable from µg to mg

• 100% recovery

Abnova have recently brought the Fastlink range of innovative antibody labelling and conjugation kits to the market. These kits allow elimination of the separation steps associated with antibody and protein labelling. The mixture of conjugate stabilizers and enzyme labels are provided in a lyophilized format. Addition of the antibody or protein solution to be labelled is required. Excess reactive chemicals then decay over several hours and by-products are benign in immunoassays.

Hands-on time for antibody conjugation is reduced to 30 seconds, and the performance of these enzyme-conjugated antibodies are as good as, or better than, those prepared with labour-intensive multistep conjugation protocols.

The overall procedure is completed within one vial, so this technique can be used to label small quantities of protein with 100% recovery, significantly minimizing the initial reagent amount and lowering development costs.

In traditional multistep protocols, 30 to 40 parameters need to be tightly controlled, impacting yield and reproducibility. Now, with the one-step labelling technology, researchers need only to control a few conjugation parameters, so the conjugation reactions are also relatively easy to scale up.

Fastlink Labelling Kits Available include:

Enzymes, Fluorescent Proteins, Fluorescent Dyes, Biotin, and Streptavidin

 

Applications include:

Western-Blotting, Immunohistochemistry, Immunofluorescence, ELISA, FACS, FRET